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This Concept Map, created with IHMC CmapTools, has information related to: CSF Legislation prescribed assays, Virologic assays ???? Virus isolation, E.U. legislation (2002/106/CE) Clinical suspicion Serologic assays, Serologic assays Number of samples Enough pigs blood samples to detect 10% prevalence with 95% confidence., 59 sows/holding if positif Blood samples from piglets to detect virus, angitgène or viral genome, E.U. legislation (2002/106/CE) Clinical suspicion Virologic assays, PCR Samples (organs/tissues) Samples size depends on the authority decision, Sows: seroprevalence of 5% with 95% C.I. Via Epi tools 59 sows/holding, E.U. legislation (2002/106/CE) If surveillance in wild boars is requested, Serologic assays ???? VNT, Serologic assays ???? ELISA, Virologic assays ???? PCR, 59 sows/holding if positif Extra serological tests, Virologic assays ???? Fluorescent antibody test, Enough pigs blood samples to detect 10% prevalence with 95% confidence. Via Epi tools 29 animals/holding, Virologic assays ???? Ag ELISA, Fluorescent antibody test Samples (organs/tissues) Samples size depends on the authority decision, Enough pigs blood samples to detect 10% prevalence with 95% confidence. Exept Semen collection centre, blood samples must be taken from all boars., Enough pigs blood samples to detect 10% prevalence with 95% confidence. Exept Sows: seroprevalence of 5% with 95% C.I., Virus isolation Samples (organs/tissues) Samples size depends on the authority decision, Ag ELISA Samples (organs/tissues) Samples size depends on the authority decision