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This Concept Map, created with IHMC CmapTools, has information related to: African Horse sickness (VF), OIE recommendations for importations Chap. 12.1.6 Chap. 12.1.8 Precribed assays for international trade Complement fixation test, Indirect/blocking ELISA Alternative PCR, Guarantee healthy animals before importation via veterinary certificate ???? General legislation, OIE prescribed assays for international trade ???? Indirect/blocking ELISA, EU official assays for AHS are Competitive ELISA, Freedom Free country or zone Theoretical sample size calculation: 116 horses (5% prev., 95% C.I.), EU official assays for AHS are Indirect ELISA, African Horse Sickness Scenarios Importation, Complement fixation test Alternative PCR, PCR Samples: uncoagulated blood, lymph node, spleen, lung Pooled samples: no Species: horses, donkeys, zebra Se:ᢒ% Sp:> 90% Detection: from 5 dpi to 10 dpi Samples/day:72(ur.), 400(c) Results (days): 5 hours (ur.), 6 (r) 1 day to swith the flow Experience in diagnotic: no Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 68.2 € If positif first samples are confirmed by Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) -> 2-3 days entire process Guard duty:no Accreditation (ISO 17025): no, PCR If positif first samples V.I., If suspicion confirmed: Dir 92/35, art 5-22 Protection zone: min. 100 km, CODA-CERVA ???? Blocking ELISA, Early detection Passive surveillance Clinical suspicion, Indirect/blocking ELISA Alternative PCR, Spread CODA-CERVA PCR, Freedom Free country or zone CODA-CERVA, Complement fixation test Alternative VNT, If suspicion confirmed: Dir 92/35, art 5-22 Control and measures during min 12 months if vaccination has been carried out, African Horse Sickness Scenarios Freedom