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This Concept Map, created with IHMC CmapTools, has information related to: Vesicular stomatitis VF, If suspicion confirmed Dir 92/119 Surveillance zone: min. 10 km, Freedom Free country or zone CODA-CERVA, Freedom Repopulation > 21 days after completion of the operations, E.U legislation Importations from third countries Animals had remained since birth or min 40 days in a area (min 10 km radius) there has been no case of VS for 40 days, Clinical suspicion CODA-CERVA Resp. K. De Klercq K. Tel : +32 (0)2/ 379 05 PCR (specific ser. New Jersey) Samples: idem V.I. Pooled samples: Species: horses, cattle, small ruminants, pigs Se.: ᢗ% Sp.: ᢗ% Detection: blood: 1-2 days before the lesions appear. During the period lesions are present Samples/day: 6-10 (ur.), 15 (c) Results (days):8 opening hours (ur.), 1 (c), 4 (horses, pigs)(r), 6 (ruminants)(r) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 80.4-96.5€, Vesicular stomatitis Diagnostic assays Commercial kits, Clinical suspicion CODA-CERVA Resp. K. De Klercq K. Tel : +32 (0)2/ 379 05 PCR (specific ser. Indiana) Samples: idem V.I. Pooled samples: Species: insects, all mammals Se.: ᢗ% Sp.: ᢗ% Detection: blood: 1-2 days before the lesions appear. During the period lesions are present Samples/day: 6-10 (ur.), 15 (c) Results (days): 8 opening hours (ur.), 1 (c), 4 (horses, pigs)(r), 6 (ruminants)(r) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 80.4-96.5€, OIE prescribed assays ???? VNT, OIE prescribed assays ???? Blocking/competitiveELISA, PCR (specific ser. New Jersey) Samples: idem V.I. Pooled samples: Species: horses, cattle, small ruminants, pigs Se.: ᢗ% Sp.: ᢗ% Detection: blood: 1-2 days before the lesions appear. During the period lesions are present Samples/day: 6-10 (ur.), 15 (c) Results (days):8 opening hours (ur.), 1 (c), 4 (horses, pigs)(r), 6 (ruminants)(r) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 80.4-96.5€ if + depending on the situation 2nd V.I. depending on the situation, OIE prescribed assays ???? CFT, Spread CODA-CERVA PCR (ser. specific), Freedom Individual certification for exportations OIE prescribed assays, VNT Samples: coagulated blood (serum) Pooled samples: Species: horses, cattle, small ruminants, pigs Se.: ᢗ% Sp.: ᢗ% Able to serotype: yes Diva: no Detection: from 7 dpi to several months dpi Samples/day: 5 (ur.) Results (days):3-4 (ur.), 16 (r) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 68.1-81.7€ if - wait 2-3 weeks 2nd VNT, Clinical suspicion CODA-CERVA Resp. K. De Klercq K. Tel : +32 (0)2/ 379 05 V.I. Samples:uncoagulated lood, coagulated blood, muccocutaneous lesions, unruptured vesicules, oropharyngeal swabs, nasal swabs, swabs from freshly ruptured vesicules Pooled samples: Species: ruminants, horses, cattle, small ruminants, pigs Se.: ᢗ% Sp.:ᢗ% Detection: blood: 1-2 days before the lesions appear. During the period lesions are present Samples/day: 6-10 (ur.), 15 (c) Results (days): 3 (ur.), 16 (r), 2(c) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample:68.1- 81.7 €, Importations Live animals Guarantee healthy animals before importation via veterinary certificate, Guarantee healthy animals before importation via veterinary certificate ???? General legislation, CODA-CERVA ???? VNT Samples: coagulated blood (serum) Pooled samples: Species: horses, cattle, small ruminants, pigs Se.: ᢗ% Sp.: ᢗ% Able to serotype: yes Diva: no Detection: from 7 dpi to several months dpi Samples/day: 5 (ur.) Results (days):3-4 (ur.), 16 (r) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 68.1-81.7€, Vesicular stomatitis Diagnostic assays Literature review, PCR (specific ser. New Jersey) Samples: idem V.I. Pooled samples: Species: horses, cattle, small ruminants, pigs Se.: ᢗ% Sp.: ᢗ% Detection: blood: 1-2 days before the lesions appear. During the period lesions are present Samples/day: 6-10 (ur.), 15 (c) Results (days):8 opening hours (ur.), 1 (c), 4 (horses, pigs)(r), 6 (ruminants)(r) 5 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 80.4-96.5€ if + depending on the situation Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) Guard duty:no Accreditation (ISO 17025): no