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This Concept Map, created with IHMC CmapTools, has information related to: Lumpy skin disease, sheep and goat pox VF, CODA-CERVA 1st year IPMA (experimental) ± PCR, Lumpy skin disease/Sheep pox/ Goat pox Diagnostic assays Literature review, OIE recommandations Pox: art. 14.9.4 to 14.9.6 LSD: art. 11.11.3 to 11.11.6 No prescribed assay Alternative: VNT, From a country indemn from LSD for min 12 months (E.U. legislation) and Animals had remained since birth or min 40 days in a area (min 10 km radius) there has been no case of LSD for 40 days, Clinical suspicion CODA-CERVA Resp K. De Klercq Tel : +32 (0)2/ 379 05 12 IPMA (capripox) Samples: uncoagulated blood (serum) Pooled samples: Species: cattle, goats, sheeps Se./Sp.: being assessed Detection: from 10 to 14 Samples/day: 60(r) to 90 (c) Results: 5 d (r/c) 7 days to switch the flow Experience in diagnotic: no Directly functional: no (experimental) Guard duty: no Accreditation (ISO 17025): no Price/sample: 81.7€, Clinical suspicion ???? EFSA prescribed assays, Multiplex PCR (capripox) Samples: skin scrabs, nodules,blood, vectors Pooled samples: no Species:cattle, goats, sheeps Se.: An. Se 3368, 3305, 4173 genome equivalents/ml.; DSe:100% Sp.:DSp: 99,38% (and 100% if PCR in parallel) No reaction with BHV 1 and 2, BTV ser.1, 2, 4, 6, 8, 9, 11 and 16 , PPRV, bovine papular stomatitis virus and contagious ecthyma virus Detection:from 2 to 8 in blood and until 10 in blood (variable ++). When skin lesions are present (from 14 dpi to...) Samples/day: 3 hours/ 20 samples.Max. 40 blood samples and 20 organs/ person/day(r)/(c) Results in 8 hours to 7 d(r); 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 32.2€ confirmation PCR, Lumpy skin disease/Sheep pox/ Goat pox Scenario Importation, IPMA (experimental) ± PCR 2nd year VNT Samples: serum Pooled samples: no Species: cattle, goats, sheeps Se./Sp.: being assessed DIVA: no Detection: ND Samples/day: 12 to 20 (r) Results (days) : 5 Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 81.7€, Multiplex PCR (capripox) Samples: skin scrabs, nodules,blood, vectors Pooled samples: no Species:cattle, goats, sheeps Se.: An. Se 3368, 3305, 4173 genome equivalents/ml.; DSe:100% Sp.:DSp: 99,38% (and 100% if PCR in parallel) No reaction with BHV 1 and 2, BTV ser.1, 2, 4, 6, 8, 9, 11 and 16 , PPRV, bovine papular stomatitis virus and contagious ecthyma virus Detection:from 2 to 8 in blood and until 10 in blood (variable ++). When skin lesions are present (from 14 dpi to...) Samples/day: 3 hours/ 20 samples.Max. 40 blood samples and 20 organs/ person/day(r)/(c) Results in 8 hours to 7 d(r); 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 32.2€ confirmation V.I. Samples: epithelium, organs Pooled samples: no Species: cattle, goats, sheeps Se./Sp.: gold standard Detection: when lesions are present Samples/day: 6 samples/person/day Results (days): 3 to 4 (c or s) to 7 (r) 7 days to switch the flow Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 81.7€, Importation Live animals OIE recommandations Pox: art. 14.9.4 to 14.9.6 LSD: art. 11.11.3 to 11.11.6, Multiplex PCR (capripox) Samples: skin scrabs, nodules,blood, vectors Pooled samples: no Species:cattle, goats, sheeps Se.: An. Se 3368, 3305, 4173 genome equivalents/ml.; DSe:100% Sp.:DSp: 99,38% (and 100% if PCR in parallel) No reaction with BHV 1 and 2, BTV ser.1, 2, 4, 6, 8, 9, 11 and 16 , PPRV, bovine papular stomatitis virus and contagious ecthyma virus Detection:from 2 to 8 in blood and until 10 in blood (variable ++). When skin lesions are present (from 14 dpi to...) Samples/day: 3 hours/ 20 samples.Max. 40 blood samples and 20 organs/ person/day(r)/(c) Results in 8 hours to 7 d(r); 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 32.2€ confirmation Sequencing (ABI 3130) Samples/day: 38 Results: 2.5 hours (only the sequencing) Guard duty:no Accreditation (17025): no, IPMA (experimental) ± PCR 2nd year VNT or IPMA, Lumpy skin disease/Sheep pox/ Goat pox Scenario Freedom, PCR Confirmation (if required) PCR, PCR Confirmation (if required) V.I., Lumpy skin disease/Sheep pox/ Goat pox Diagnostic assays Commercial kits, Clinical suspicion CODA-CERVA Resp K. De Klercq Tel : +32 (0)2/ 379 05 12 Multiplex PCR (capripox) Samples: skin scrabs, nodules,blood, vectors Pooled samples: no Species:cattle, goats, sheeps Se.: An. Se 3368, 3305, 4173 genome equivalents/ml.; DSe:100% Sp.:DSp: 99,38% (and 100% if PCR in parallel) No reaction with BHV 1 and 2, BTV ser.1, 2, 4, 6, 8, 9, 11 and 16 , PPRV, bovine papular stomatitis virus and contagious ecthyma virus Detection:from 2 to 8 in blood and until 10 in blood (variable ++). When skin lesions are present (from 14 dpi to...) Samples/day: 3 hours/ 20 samples.Max. 40 blood samples and 20 organs/ person/day(r)/(c) Results in 8 hours to 7 d(r); 1 (c) Experience in diagnotic: yes Directly functional: yes Guard duty: no Accreditation (ISO 17025): no Price/sample: 32.2€, Importation Live animals From a country indemn from LSD for min 12 months (E.U. legislation), Spread CODA-CERVA PCR